Identification of Leishmania species by high-resolution DNA dissociation in cases of American cutaneous leishmaniasis
نویسندگان
چکیده
منابع مشابه
Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran
Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various species of Leishmania, each having a different secondary host. Thus, identifying the parasites’ specie is of paramount importance for containment strategy planning. The morpho...
متن کاملDetermination of Leishmania species causing cutaneous leishmaniasis in Mashhad by PCR-RFLP method
Three species of L. tropica, L. major and L. infantum are known as main causal agents of leishmaniasis have been reported in Iran. Since cutaneous leishmaniasis (CL) is endemic in North East of Iran, in the present work, 50 Leishmania positive isolates from human cases in Mashhad (Center of Razavi province, North East of Iran), were genotyped by means of polymerase chain reaction-restriction f...
متن کاملIdentification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran
ABSTRACT Background and Objective: Chabahar is in Southern Iran located near the Iran-Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of Leishmania parasite in the patients referred to the central laboratory. Methods: Thi...
متن کاملidentification of leishmania species isolated from human cutaneous leishmaniasis, using random amplified polymorphic dna (rapd-pcr)
this cross-sectional study was designed to isolate of leishmania spp from cutaneous leishmaniasis patients and characterized them by rapd-pcr technique. eighty- seven leishmania isolates from 112 samples were collected from cutaneous leishmaniasis (cl) patients who referred to mashhad health centers from august 2002 to may 2004. desirable samples (87 isolates) were characterized by rapd-pcr met...
متن کاملDetection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis.
BACKGROUND Traditional detection of Leishmania from ulcers involves collection of invasive specimens that cause discomfort, require technical expertise, and carry risks of invasive procedures. We compared traditional diagnostic methods with a molecular noninvasive filter paper-based method for the diagnosis of cutaneous leishmaniasis. METHODS Consecutive patients presenting to the Leishmania ...
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ژورنال
عنوان ژورنال: Anais Brasileiros de Dermatologia
سال: 2020
ISSN: 0365-0596
DOI: 10.1016/j.abd.2020.02.003